recombinant human galectin (Galectin Therapeutics)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Galectin Therapeutics recombinant human galectin

(A) Galectin-family protein groups, including an LGALS13-annotated / <t>CLC-Galectin-10-like</t> porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

Recombinant Human Galectin, supplied by Galectin Therapeutics, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/recombinant+human+galectin/lgals3/bio_rxiv__64898__2026__05__16__725632-71-0-3
Average 86 stars, based on 1 article reviews

recombinant human galectin - by Bioz Stars, 2026-10

86/100 stars

Images

1) Product Images from "Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression"

Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression

Journal: bioRxiv

doi: 10.64898/2026.05.16.725632

(A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

Figure Legend Snippet: (A) Galectin-family protein groups, including an LGALS13-annotated / CLC-Galectin-10-like porcine orthologous protein group. (B) Immune lineage-associated proteins (T-cell, B-cell, myeloid, cytotoxic, and pan-leukocyte markers). (C) Selected signaling protein groups (NF-κB and JAK/STAT axes). DIA proteomics was performed on effluent cell pellets from one representative biological perfusion in technical triplicate and should be interpreted as exploratory cell-associated protein abundance. Values are log2 fold-changes relative to the Pre-1 baseline; heatmap scale capped at ±3 for display. UD = undetected.

Techniques Used: Quantitative Proteomics

$Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.$
Figure Legend Snippet: Jurkat T cells were stimulated with anti-CD3/CD28 (5 µg/mL) for 1 h before addition of recombinant human Galectin-10 or Galectin-13 (20 µg/mL) for 24 h. Cells were stained with Annexin V and propidium iodide (PI) and analyzed by flow cytometry. Stacked bars decompose the total Annexin V+ population into early-apoptotic (Annexin V+ / PI−, gold) and late-apoptotic / dying (Annexin V+ / PI+, red) fractions. Total Annexin V+ values are annotated above each bar; error bars represent SEM on the total. Total Annexin V+ values were compared across conditions by one-way ANOVA with Dunnett’s multiple-comparison test against the stimulated control. Individual replicate values are shown as overlaid points (n = 3 per condition). Human Galectin-10 produced ∼84% total Annexin V+ positivity, the great majority of which had progressed to the late-apoptotic / dying state by 24 h compared with stimulated alone (p<0.001). Galectin-13 produced a more modest increase. This assay was selected because the porcine LGALS13-annotated proteomic signal is interpreted as a CLC/Galectin-10-like orthologous axis; it supports prioritization of that axis but does not prove porcine protein identity or causality in the perfused spleen.

Techniques Used: Recombinant, Staining, Flow Cytometry, Comparison, Control, Produced

Recombinant:

Article Title: Immunoengineering can overcome the glycocalyx armor of cancer cells
Article Snippet: KO of C1GALT1 in the 1E7 cells was achieved using the Alt-R CRISPR/Cas9 system (Integrated DNA Technologies) with HDR templates that introduced an in-frame stop codon and an EcoRI restriction site for screening (See Supplementary Table 3 ). .. 10 μM recombinant human galectin-1 was incubated with 1E7 GCNT1 OE cells for 10 minutes at 37°C. .. Intensity profiles were corrected by normalizing to the time-course decay of GFP signal in non-bleached area and analyzed by curve-fitting to calculate the half time of recovery (T 1/2 ) and mobile fraction using MATLAB code available from the Janelia Advanced Imaging Center ( https://www.mathworks.com/matlabcentral/fileexchange/47327-frap-zip ).

Article Title: Mucins form a nanoscale material barrier against immune cell attack
Article Snippet: .. 10 μM recombinant human galectin-1 was incubated with 1E7 GCNT1 cells for 10 752 minutes at 37oC (n=9). .. Intensity profiles were corrected by normalizing to the time-course decay 753 of GFP signal in non-bleached area by using MATLAB code from Advanced Imaging Center 754 (https://www.mathworks.com/matlabcentral/fileexchange/47327-frap-zip) 755 Scanning Angle Interference Microscopy 756 Sample preparation 757 Silicon wafers with ~1900 nm thermal oxide (Addison Engineering) were diced into 7 mm by 7 758 mm chips, and the oxide layer thickness for each chip was measured with a FilMetrics F50-EXR.

Article Title: Dose-Response Models Reveal Critical Features of Inhibitor Activity and Viral Infection
Article Snippet: 75 Galectin-9 treatment and flow cytometry. .. Recombinant human Galectin-9 was added to cells in culture to achieve a final concentration of 0.1μM after which cells were incubated for 2 hours at 37◦C. .. All flow staining samples (Galectin-9 treated and untreated) were washed twice in PBS supplemented with 10% (v/v) fetal bovine serum and stained for 1 hour at 4◦C with 1:100X RL77 (PDI), Gal-9 antibody or 0.5mM biotin maleimide.

Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression
Article Snippet: CD3/CD28-stimulated Jurkat cells were treated with recombinant human Galectin-10 or Galectin-13 at 20 μg/mL for 24 h, and apoptosis/cell death was measured by Annexin V and propidium iodide (PI) staining. .. – Recombinant human Galectin-10 produced a marked increase in Annexin V positivity and Annexin V/PI double-positivity. .. Annexin V+ cells increased from 8.0 ± 0.3% in resting cells and 19.4 ± 0.9% in stimulated controls to 84.3 ± 7.2% with human Galectin-10.

Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression
Article Snippet: In parallel, data independent acquisition (DIA) proteomics of effluent cell pellets nominated a non-transcriptional protein-level response: a Sus scrofa LGALS13-annotated, CLC/Galectin-10-like galectin detected despite absence of the corresponding effluent-cell transcript. .. Because this porcine LGALS13-annotated protein group is treated here as an orthologous CLC/Galectin-10-like signal rather than as canonical human placental Galectin-13/PP13, we tested recombinant human Galectin-10 in vitro. .. Human Galectin-10 induced marked apoptosis of CD3/CD28-stimulated Jurkat cells, prioritizing this axis for future mechanistic testing without proving causality in the perfused spleen.

Article Title: Galectin-1, an endogenous lectin produced by thymic epithelial cells, induces apoptosis of human thymocytes.
Article Snippet: Our data is consistent with a role for galectin-1 in the induction of apoptosis of negatively selected and nonselected thymocytes. .. Preparation of Recombinant Human Galectin-1. .. Galectin-1 was prepared by the method of Couraud et al. (23) in Escherichia coli strain BL21 (DE3) transformed with the expression vector pT7IML-1 (gift of Incyte Pharmaceuticals, Inc., Palo Alto, CA).

Incubation:

Concentration Assay:

Produced:

In Vitro:

Article Title: Acellular normothermic spleen perfusion resolves transcriptional and non-transcriptional mechanisms of steroid immunosuppression
Article Snippet: In parallel, data independent acquisition (DIA) proteomics of effluent cell pellets nominated a non-transcriptional protein-level response: a Sus scrofa LGALS13-annotated, CLC/Galectin-10-like galectin detected despite absence of the corresponding effluent-cell transcript. .. Because this porcine LGALS13-annotated protein group is treated here as an orthologous CLC/Galectin-10-like signal rather than as canonical human placental Galectin-13/PP13, we tested recombinant human Galectin-10 in vitro. .. Human Galectin-10 induced marked apoptosis of CD3/CD28-stimulated Jurkat cells, prioritizing this axis for future mechanistic testing without proving causality in the perfused spleen.